Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?
Identifieur interne : 000563 ( Main/Exploration ); précédent : 000562; suivant : 000564Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?
Auteurs : V. Chovelon [France] ; V. Restier ; N. Giovinazzo ; C. Dogimont ; J. AarroufSource :
- Plant cell reports [ 1432-203X ] ; 2011.
Descripteurs français
- KwdFr :
- Agrobacterium (effets des médicaments et des substances chimiques), Agrobacterium (physiologie), Cucumis melo (anatomie et histologie), Cucumis melo (cytologie), Cucumis melo (embryologie), Cucumis melo (génétique), Glucuronidase (métabolisme), Génotype (MeSH), Milieux de culture (pharmacologie), Organogenèse (effets des médicaments et des substances chimiques), Organogenèse (génétique), Régénération (effets des médicaments et des substances chimiques), Régénération (génétique), Transformation génétique (effets des médicaments et des substances chimiques), Végétaux génétiquement modifiés (MeSH).
- MESH :
- anatomie et histologie : Cucumis melo.
- cytologie : Cucumis melo.
- effets des médicaments et des substances chimiques : Agrobacterium, Organogenèse, Régénération, Transformation génétique.
- embryologie : Cucumis melo.
- génétique : Cucumis melo, Organogenèse, Régénération.
- métabolisme : Glucuronidase.
- pharmacologie : Milieux de culture.
- physiologie : Agrobacterium.
- Génotype, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium (drug effects), Agrobacterium (physiology), Cucumis melo (anatomy & histology), Cucumis melo (cytology), Cucumis melo (embryology), Cucumis melo (genetics), Culture Media (pharmacology), Genotype (MeSH), Glucuronidase (metabolism), Organogenesis (drug effects), Organogenesis (genetics), Plants, Genetically Modified (MeSH), Regeneration (drug effects), Regeneration (genetics), Transformation, Genetic (drug effects).
- MESH :
- chemical , metabolism : Glucuronidase.
- chemical , pharmacology : Culture Media.
- anatomy & histology : Cucumis melo.
- cytology : Cucumis melo.
- drug effects : Agrobacterium, Organogenesis, Regeneration, Transformation, Genetic.
- embryology : Cucumis melo.
- genetics : Cucumis melo, Organogenesis, Regeneration.
- physiology : Agrobacterium.
- Genotype, Plants, Genetically Modified.
Abstract
Melon (Cucumis melo L.) is widely considered as a recalcitrant species for genetic transformation. In this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. The highest regeneration rate (1.13 ± 0.02 plants per explant) was obtained using cotyledon explants of the 'Védrantais' genotype on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l dimethylallylaminopurine (2-iP). Agrobacterium tumefaciens-mediated transformations with the uidA reporter gene were realized on cotyledon explants cultivated in these conditions: 70-90% of explants expressed a transient GUS activity during the early stages of regeneration, however, only few transgenic plants were obtained (1.8-4.5% of stable transformation with the GV2260pBI101 strain). These results revealed a low capacity of melon GUS-positive cells to regenerate transgenic plants. To evaluate the influence of the Agrobacterium infection on plant regeneration, histological analyses were conducted on explants 2, 7, 15, and 28 days after co-culture with the GV2260pBI101 strain. Genetic transformation occurred in epidermal and sub-epidermal cells and reached the meristematic structures expressing a high level of GUS activity during 14 days of culture; but after this period, most of the meristematic structures showed premature cell vacuolization and disorganization. This disruption of the GUS-positive meristematic areas could be responsible of the difficulties encountered to regenerate melon plants after genetic transformation.
DOI: 10.1007/s00299-011-1108-9
PubMed: 21706229
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?</title><author><name sortKey="Chovelon, V" sort="Chovelon, V" uniqKey="Chovelon V" first="V" last="Chovelon">V. Chovelon</name><affiliation wicri:level="3"><nlm:affiliation>INRA Avignon, UR1052, Unité de Génétique et d'Amélioration des Fruits et Légumes, BP 94, 84143, Montfavet Cedex, France. Veronique.chovelon@avignon.inra.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA Avignon, UR1052, Unité de Génétique et d'Amélioration des Fruits et Légumes, BP 94, 84143, Montfavet Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Montfavet</settlement></placeName></affiliation></author><author><name sortKey="Restier, V" sort="Restier, V" uniqKey="Restier V" first="V" last="Restier">V. Restier</name></author><author><name sortKey="Giovinazzo, N" sort="Giovinazzo, N" uniqKey="Giovinazzo N" first="N" last="Giovinazzo">N. Giovinazzo</name></author><author><name sortKey="Dogimont, C" sort="Dogimont, C" uniqKey="Dogimont C" first="C" last="Dogimont">C. Dogimont</name></author><author><name sortKey="Aarrouf, J" sort="Aarrouf, J" uniqKey="Aarrouf J" first="J" last="Aarrouf">J. Aarrouf</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21706229</idno><idno type="pmid">21706229</idno><idno type="doi">10.1007/s00299-011-1108-9</idno><idno type="wicri:Area/Main/Corpus">000556</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000556</idno><idno type="wicri:Area/Main/Curation">000556</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000556</idno><idno type="wicri:Area/Main/Exploration">000556</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?</title><author><name sortKey="Chovelon, V" sort="Chovelon, V" uniqKey="Chovelon V" first="V" last="Chovelon">V. Chovelon</name><affiliation wicri:level="3"><nlm:affiliation>INRA Avignon, UR1052, Unité de Génétique et d'Amélioration des Fruits et Légumes, BP 94, 84143, Montfavet Cedex, France. Veronique.chovelon@avignon.inra.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA Avignon, UR1052, Unité de Génétique et d'Amélioration des Fruits et Légumes, BP 94, 84143, Montfavet Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Montfavet</settlement></placeName></affiliation></author><author><name sortKey="Restier, V" sort="Restier, V" uniqKey="Restier V" first="V" last="Restier">V. Restier</name></author><author><name sortKey="Giovinazzo, N" sort="Giovinazzo, N" uniqKey="Giovinazzo N" first="N" last="Giovinazzo">N. Giovinazzo</name></author><author><name sortKey="Dogimont, C" sort="Dogimont, C" uniqKey="Dogimont C" first="C" last="Dogimont">C. Dogimont</name></author><author><name sortKey="Aarrouf, J" sort="Aarrouf, J" uniqKey="Aarrouf J" first="J" last="Aarrouf">J. Aarrouf</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (drug effects)</term><term>Agrobacterium (physiology)</term><term>Cucumis melo (anatomy & histology)</term><term>Cucumis melo (cytology)</term><term>Cucumis melo (embryology)</term><term>Cucumis melo (genetics)</term><term>Culture Media (pharmacology)</term><term>Genotype (MeSH)</term><term>Glucuronidase (metabolism)</term><term>Organogenesis (drug effects)</term><term>Organogenesis (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Regeneration (drug effects)</term><term>Regeneration (genetics)</term><term>Transformation, Genetic (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium (effets des médicaments et des substances chimiques)</term><term>Agrobacterium (physiologie)</term><term>Cucumis melo (anatomie et histologie)</term><term>Cucumis melo (cytologie)</term><term>Cucumis melo (embryologie)</term><term>Cucumis melo (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Génotype (MeSH)</term><term>Milieux de culture (pharmacologie)</term><term>Organogenèse (effets des médicaments et des substances chimiques)</term><term>Organogenèse (génétique)</term><term>Régénération (effets des médicaments et des substances chimiques)</term><term>Régénération (génétique)</term><term>Transformation génétique (effets des médicaments et des substances chimiques)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Cucumis melo</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Cucumis melo</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cucumis melo</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Cucumis melo</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Agrobacterium</term><term>Organogenesis</term><term>Regeneration</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Agrobacterium</term><term>Organogenèse</term><term>Régénération</term><term>Transformation génétique</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Cucumis melo</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Cucumis melo</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cucumis melo</term><term>Organogenesis</term><term>Regeneration</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cucumis melo</term><term>Organogenèse</term><term>Régénération</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Agrobacterium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genotype</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Génotype</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Melon (Cucumis melo L.) is widely considered as a recalcitrant species for genetic transformation. In this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. The highest regeneration rate (1.13 ± 0.02 plants per explant) was obtained using cotyledon explants of the 'Védrantais' genotype on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l dimethylallylaminopurine (2-iP). Agrobacterium tumefaciens-mediated transformations with the uidA reporter gene were realized on cotyledon explants cultivated in these conditions: 70-90% of explants expressed a transient GUS activity during the early stages of regeneration, however, only few transgenic plants were obtained (1.8-4.5% of stable transformation with the GV2260pBI101 strain). These results revealed a low capacity of melon GUS-positive cells to regenerate transgenic plants. To evaluate the influence of the Agrobacterium infection on plant regeneration, histological analyses were conducted on explants 2, 7, 15, and 28 days after co-culture with the GV2260pBI101 strain. Genetic transformation occurred in epidermal and sub-epidermal cells and reached the meristematic structures expressing a high level of GUS activity during 14 days of culture; but after this period, most of the meristematic structures showed premature cell vacuolization and disorganization. This disruption of the GUS-positive meristematic areas could be responsible of the difficulties encountered to regenerate melon plants after genetic transformation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21706229</PMID><DateCompleted><Year>2012</Year><Month>02</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>11</Issue><PubDate><Year>2011</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?</ArticleTitle><Pagination><MedlinePgn>2001-11</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-011-1108-9</ELocationID><Abstract><AbstractText>Melon (Cucumis melo L.) is widely considered as a recalcitrant species for genetic transformation. In this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. The highest regeneration rate (1.13 ± 0.02 plants per explant) was obtained using cotyledon explants of the 'Védrantais' genotype on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l dimethylallylaminopurine (2-iP). Agrobacterium tumefaciens-mediated transformations with the uidA reporter gene were realized on cotyledon explants cultivated in these conditions: 70-90% of explants expressed a transient GUS activity during the early stages of regeneration, however, only few transgenic plants were obtained (1.8-4.5% of stable transformation with the GV2260pBI101 strain). These results revealed a low capacity of melon GUS-positive cells to regenerate transgenic plants. To evaluate the influence of the Agrobacterium infection on plant regeneration, histological analyses were conducted on explants 2, 7, 15, and 28 days after co-culture with the GV2260pBI101 strain. Genetic transformation occurred in epidermal and sub-epidermal cells and reached the meristematic structures expressing a high level of GUS activity during 14 days of culture; but after this period, most of the meristematic structures showed premature cell vacuolization and disorganization. This disruption of the GUS-positive meristematic areas could be responsible of the difficulties encountered to regenerate melon plants after genetic transformation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chovelon</LastName><ForeName>V</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>INRA Avignon, UR1052, Unité de Génétique et d'Amélioration des Fruits et Légumes, BP 94, 84143, Montfavet Cedex, France. Veronique.chovelon@avignon.inra.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Restier</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Giovinazzo</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Dogimont</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Aarrouf</LastName><ForeName>J</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>06</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028463" MajorTopicYN="N">Cucumis melo</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000196" MajorTopicYN="N">embryology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038081" MajorTopicYN="N">Organogenesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012038" MajorTopicYN="N">Regeneration</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>02</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>06</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>6</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>6</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21706229</ArticleId><ArticleId IdType="doi">10.1007/s00299-011-1108-9</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell. 2007 May;19(5):1695-708</Citation><ArticleIdList><ArticleId IdType="pubmed">17496122</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1997 Mar;9(3):317-33</Citation><ArticleIdList><ArticleId IdType="pubmed">9090878</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2006 Mar;25(3):198-205</Citation><ArticleIdList><ArticleId IdType="pubmed">16362302</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2007 Sep;20(9):1048-54</Citation><ArticleIdList><ArticleId IdType="pubmed">17849707</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Lett. 2011 Jan;33(1):55-61</Citation><ArticleIdList><ArticleId IdType="pubmed">20865442</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Mol Biol Int. 1998 Dec;46(6):1201-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9891853</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Issues Mol Biol. 2001 Apr;3(2):27-34</Citation><ArticleIdList><ArticleId IdType="pubmed">11471972</ArticleId></ArticleIdList></Reference><Reference><Citation>Crit Rev Biotechnol. 2008;28(1):13-55</Citation><ArticleIdList><ArticleId IdType="pubmed">18322855</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Mol Biol Rev. 2003 Mar;67(1):16-37, table of contents</Citation><ArticleIdList><ArticleId IdType="pubmed">12626681</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1991 Jul;96(3):985-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16668285</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Oct 22;461(7267):1135-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19847267</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1983;101:433-81</Citation><ArticleIdList><ArticleId IdType="pubmed">6577258</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 1997 Jan;6(1):41-50</Citation><ArticleIdList><ArticleId IdType="pubmed">9032977</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1996 Jul;14(7):862-6</Citation><ArticleIdList><ArticleId IdType="pubmed">9631011</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2005 Mar;23(10-11):692-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15480686</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2000 Oct;5(10):446-51</Citation><ArticleIdList><ArticleId IdType="pubmed">11044722</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 Jul;35(2):219-36</Citation><ArticleIdList><ArticleId IdType="pubmed">12848827</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1990 Jul;9(3):160-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24226603</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2000 Jun;13(6):649-57</Citation><ArticleIdList><ArticleId IdType="pubmed">10830264</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1977 Mar;59(3):388-90</Citation><ArticleIdList><ArticleId IdType="pubmed">16659858</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 2009 Jan 1;139(1):1-5</Citation><ArticleIdList><ArticleId IdType="pubmed">18831993</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1993 May-Jun;6(3):358-67</Citation><ArticleIdList><ArticleId IdType="pubmed">8324251</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Montfavet</li></settlement></list><tree><noCountry><name sortKey="Aarrouf, J" sort="Aarrouf, J" uniqKey="Aarrouf J" first="J" last="Aarrouf">J. Aarrouf</name><name sortKey="Dogimont, C" sort="Dogimont, C" uniqKey="Dogimont C" first="C" last="Dogimont">C. Dogimont</name><name sortKey="Giovinazzo, N" sort="Giovinazzo, N" uniqKey="Giovinazzo N" first="N" last="Giovinazzo">N. Giovinazzo</name><name sortKey="Restier, V" sort="Restier, V" uniqKey="Restier V" first="V" last="Restier">V. Restier</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Chovelon, V" sort="Chovelon, V" uniqKey="Chovelon V" first="V" last="Chovelon">V. Chovelon</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000563 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000563 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21706229
|texte= Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21706229" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |